Invitrogen SuperScript II Reverse Transcriptas

hiscript ii reverse transcriptase

hiscript ii reverse transcriptase

Invitrogen SuperScript II Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) with reduced RNase H activity and increased thermal stability compared to wild-type MMLV RT. Mutations in the RNase H domain of the enzyme eliminate degradation of the RNA during first-strand cDNA synthesis, which results in higher yields of full-length cDNA.

SuperScript RTs are the most highly trusted and widely used RTs with over 50,000 citations, reviews, and publications to date.

Note: The latest member of the SuperScript RT family, SuperScript IV Reverse Transcriptase, features enhanced thermostability, processivity, yields, and performance with any RNA samples, including those of suboptimal purity or integrity.

Introduction

HiScript II Q RT SuperMix for qPCR The Vazyme HiScript II Reverse Transcriptase, optimized from M-MLV (RNase H-) Reverse Transcriptase, is a new generation reverse transcriptase with highly improved heat stability and cDNA synthesis efficiency. The HiScript II Q RT SuperMix for qPCR is specially designed for 2-step RT-qPCR. The 5× Mix
contains all necessary components needed for reverse transcription, including Buffer, dNTPs, HiScript II Reverse Transcriptase, RNase inhibitor, and Random primers/Oligo-dT primer mix.
The HiScript II Q RT SuperMix for qPCR has been specially optimized for qPCR. For example, the ratio of Random primers/Oligo-dT primer is optimized to enable cDNA synthesis at any region of the template RNA and to ensure the repeatability of qPCR results. The cDNA products are compatible for SYBR- or probe-based qPCR, such as AceQ qPCR SYBR Green Master Mix (Vazyme, #Q111), ChamQ SYBR qPCR Master Mix (Vazyme, #Q311), ChamQ Color
SYBR qPCR Master Mix (Vazyme, #Q411), and AceQ qPCR Probe Master Mix (Vazyme, #Q112).

HiScript II Reverse Transcriptase is a new reverse transcriptase obtained through in vitro molecular evolution technology on the basis of M-MLV (RNase H-) Reverse Transcriptase. Compared with the previous generation HiScript Reverse Transcriptase, HiScript II Transcriptase has further greatly improved thermal stability, with a half-life of more than 4 hours at 50°C, and can be reacted for a long time at 55°C to maintain stability. It is very suitable for complex secondary Reverse transcription of structural RNA template.

In addition, HiScript II Transcriptase adds a number of point mutations, which further enhances template affinity and progression, greatly improving the synthesis ability of full-length cDNA, and obtaining cDNA up to 20 kb. It has a higher tolerance to common reverse transcription inhibitors and is very suitable for the reverse transcription reaction of plant tissue RNA rich in polysaccharides and polyphenols.

 

The half-life at 50℃ exceeds 240 min, and the half-life at 55℃ can reach 60 min;
CDNA up to 10 kb can be synthesized in 5 minutes, which is suitable for rapid reverse transcription reaction;
Higher impurity tolerance and higher detection sensitivity, reverse transcriptase is the first choice for molecular diagnosis.

 

hiscript ii reverse transcriptase
hiscript ii reverse transcriptase

 

Specifications

Enzyme Function
RNA dependent DNA Polymerase
Includes
SuperScript II Reverse Transcriptase (2,000 units total, at 200U/μL), 5X first-strand buffer [250mM Tris-HCl (pH 8.3), 375mM KCl, 15mM MgCl2], and 100mM DTT
No. of Tests
10 Reactions
Optimal Reaction Temperature
42° C
Product Line
SuperScript
Product Type
Reverse Transcriptase
Quantity
2000 units
Reverse Transcriptase
SuperScript II
Ribonuclease H Activity
Reduced
Shipping Condition
Wet Ice
Sufficient For
10 Reactions
Concentration
200 U⁄µl
Form
Frozen
Format
Tube(s)
GC-Rich PCR Performance
High
No. of Reactions
10 Reactions
PCR Method
RT-PCR
Size (Final Product)
12.3 kb or less
Volume
200 μL

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