Thermo Scientific Genomic DNA Purification Kit is a simple and rapid system for high quality genomic DNA purification from various sources including: whole blood, serum, cell lines, bacterial cells, plant, and mammalian tissues.
The kit is based on selective detergent-mediated DNA precipitation from crude lysate. The entire procedure is rapid only 20 to 25 minutes with a typical yield of 2 to 10 µg genomic DNA from 0.2 mL of blood.
High molecular weight genomic DNA purified with the kit is suitable for direct use in all common molecular biology applications: PCR, FastDigest and conventional restriction digestion, cloning, DNA sequencing, and Southern blot analysis.
Highlights
• Efficient—2 to 10 µg of genomic DNA from 0.2 mL of blood • Fast—procedure takes approximately 20 minutes • Universal—purifies genomic DNA from various sources • Flexible—easy to scale up and down • Safe—does not involve phenol extraction
Applications
• Purification of high-quality genomic DNA from: • Whole blood or serum • Cell culture • Plant tissues • Mammalian tissues • Epithelium samples • Bacteria Includes Lysis Solution Precipitation Solution NaCl Solution Detailed Protocol.
Genomic DNA extraction poses a number of challenges due to the wide range of potential starting materials (plant, animal and human), the consistently high yields you need and the variety of downstream applications. Our genomic DNA extraction kits overcome these challenges by enabling reproducible genomic DNA isolation from a range of sample types using optimized protocols. You can obtain high yields of high-quality DNA, even from specialized samples. The genomic purification kits are available in both bead-based and spin-column formats to suit your needs.
biolabmix du-50 genomic dna extraction kit cells
Abstract
Isolation of DNA from blood and buccal swabs in adequate quantities is an integral part of forensic research and analysis. The present study was performed to determine the quality and the quantity of DNA extracted from four commonly available samples and to estimate the time duration of the ensuing PCR amplification. Here, we demonstrate that hair and urine samples can also become an alternate source for reliably obtaining a small quantity of PCR-ready DNA. We developed a rapid, cost-effective, and noninvasive method of sample collection and simple DNA extraction from buccal swabs, urine, and hair using the phenol-chloroform method. Buccal samples were subjected to DNA extraction, immediately or after refrigeration (4–6°C) for 3 days.
The purity and the concentration of the extracted DNA were determined spectrophotometerically, and the adequacy of DNA extracts for the PCR-based assay was assessed by amplifying a 1030-bp region of the mitochondrial D-loop. Although DNA from all the samples was suitable for PCR, the blood and hair samples provided a good quality DNA for restriction analysis of the PCR product compared with the buccal swab and urine samples. In the present study, hair samples proved to be a good source of genomic DNA for PCR-based methods. Hence, DNA of hair samples can also be used for the genomic disorder analysis in addition to the forensic analysis as a result of the ease of sample collection in a noninvasive manner, lower sample volume requirements, and good storage capability.
