Genomic DNA Purification Kit

biolabmix du-50 genomic dna extraction kit cells

biolabmix du-50 genomic dna extraction kit cells

Thermo Scientific Genomic DNA Purification Kit is a simple and rapid system for high quality genomic DNA purification from various sources including: whole blood, serum, cell lines, bacterial cells, plant, and mammalian tissues.

The kit is based on selective detergent-mediated DNA precipitation from crude lysate. The entire procedure is rapid only 20 to 25 minutes with a typical yield of 2 to 10 µg genomic DNA from 0.2 mL of blood.

High molecular weight genomic DNA purified with the kit is suitable for direct use in all common molecular biology applications: PCR, FastDigest and conventional restriction digestion, cloning, DNA sequencing, and Southern blot analysis.

Highlights

• Efficient—2 to 10 µg of genomic DNA from 0.2 mL of blood
• Fast—procedure takes approximately 20 minutes
• Universal—purifies genomic DNA from various sources
• Flexible—easy to scale up and down
• Safe—does not involve phenol extraction

Applications

• Purification of high-quality genomic DNA from:
• Whole blood or serum
• Cell culture
• Plant tissues
• Mammalian tissues
• Epithelium samples
• Bacteria Includes Lysis Solution Precipitation Solution NaCl Solution Detailed Protocol.

 

Genomic DNA extraction poses a number of challenges due to the wide range of potential starting materials (plant, animal and human), the consistently high yields you need and the variety of downstream applications. Our genomic DNA extraction kits overcome these challenges by enabling reproducible genomic DNA isolation from a range of sample types using optimized protocols. You can obtain high yields of high-quality DNA, even from specialized samples. The genomic purification kits are available in both bead-based and spin-column formats to suit your needs.

 

biolabmix du-50 genomic dna extraction kit cells
biolabmix du-50 genomic dna extraction kit cells

Abstract

Isolation of DNA from blood and buccal swabs in adequate quantities is an integral part of forensic research and analysis. The present study was performed to determine the quality and the quantity of DNA extracted from four commonly available samples and to estimate the time duration of the ensuing PCR amplification. Here, we demonstrate that hair and urine samples can also become an alternate source for reliably obtaining a small quantity of PCR-ready DNA. We developed a rapid, cost-effective, and noninvasive method of sample collection and simple DNA extraction from buccal swabs, urine, and hair using the phenol-chloroform method. Buccal samples were subjected to DNA extraction, immediately or after refrigeration (4–6°C) for 3 days.

The purity and the concentration of the extracted DNA were determined spectrophotometerically, and the adequacy of DNA extracts for the PCR-based assay was assessed by amplifying a 1030-bp region of the mitochondrial D-loop. Although DNA from all the samples was suitable for PCR, the blood and hair samples provided a good quality DNA for restriction analysis of the PCR product compared with the buccal swab and urine samples. In the present study, hair samples proved to be a good source of genomic DNA for PCR-based methods. Hence, DNA of hair samples can also be used for the genomic disorder analysis in addition to the forensic analysis as a result of the ease of sample collection in a noninvasive manner, lower sample volume requirements, and good storage capability.

 

Stool DNA Purification Kit

K1474-250 250 Preps
EUR 601

Stool DNA Purification Kit

K1474-50 50 Preps
EUR 348

Mitochondrial DNA Purification Kit

K389-25
EUR 392

Zenoquick Genomic DNA Purification Kit

Z2003-200 200 preps
EUR 631

Zenoprep Genomic DNA Purification Kit

Z2004-400 400 preps
EUR 550

Zenoprep Serum DNA Purification Kit

Z2005-000 Adjustable
EUR 479

Zenoquick Viral DNA Purification Kit

Z2009-050 50 preps
EUR 340

Zenoquick Viral DNA Purification Kit

Z2009-200 4x50 preps
EUR 941

Bacterial DNA Purification Kit II

K1457-250 250 Preps
EUR 581

Bacterial DNA Purification Kit II

K1457-50 50 Preps
EUR 318

Buccal Swab DNA Purification Kit

K1466-250 250 Preps
EUR 551

Buccal Swab DNA Purification Kit

K1466-50 50 Preps
EUR 318

Zenoquick Soil Microbe DNA Purification Kit

Z2006-050 50 preps
EUR 479

PCR DNA extraction and purification kit

K1444-100
EUR 338

Plasmid DNA extraction and purification kit

K1445-100
EUR 370

Viral DNA extraction and purification kit

K1446-100
EUR 479

Gel and PCR DNA Purification kit

K1455-250 250 Preps
EUR 480

Gel and PCR DNA Purification kit

K1455-50 50 Preps
EUR 206

Dried Body Fluids DNA Purification Kit

K1464-250 250 Preps
EUR 551

Dried Body Fluids DNA Purification Kit

K1464-50 50 Preps
EUR 318

Hair, Nails & Feathers DNA Purification Kit

K1469-250 250 Preps
EUR 551

Hair, Nails & Feathers DNA Purification Kit

K1469-50 50 Preps
EUR 318

RNA/DNA Purification Kit (Magnetic Bead)

DA0623 32 test/kit Ask for price

100 BP DNA LADDER, 500UL PER KIT

M-DNA-100BP 1/pk
EUR 73
Description: Bioscience Mol Bio; DNA Ladder

Related Post

Leave a Reply

Your email address will not be published.