Thermo Scientific GeneJET Gel Extraction and DNA Cleanup Micro Kit is developed as 3 in 1 kit designed for rapid and efficient purification of DNA from PCR, enzymatic reaction mixtures, and DNA extraction from standard or low-melting point agarose gels run in either Tris acetate (TAE) or Tris borate (TBE) buffer. The kit combines the convenience of spin column technology with the selective binding properties of a silica membrane, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.
The GeneJET Gel Extraction and DNA Cleanup Micro Kit effectively removes primers, primer dimers, dNTPs, unincorporated labeled nucleotides, enzymes and salts from PCR and other reaction mixtures. The kit can be used for purification of DNA fragments from 100 bp to 20 kb. The recovery rates are 90 to 100% for 100 bp to 3 kb DNA fragment size range. Each GeneJET DNA Purification Column has a total binding capacity of up to 10 µg of DNA, and the entire procedure takes approximately 3.5 minutes for DNA cleanup
Highlights
• Advanced column design—easy to open, load and handle • Yields concentrated DNA – Elute in 6 to 10 µL volume • High recovery – 90 to 100% recovery in the 100 to 3000 bp DNA fragment range • Fast—4 minute reaction mixture clean-up and 15 minute agarose gel purification protocols available • Efficient removal of reaction contaminants – including enzymes, proteins, primers dimers, dNTPs, and salts • Flexible—does not require precise gel slice quantification weighting
Applications
The purified DNA can be used in common downstream applications:
The Invisorb® DNA CleanUp provides a convenient tool for fast and efficient cleanup of strong contaminated DNA, DNA
fragments and PCR products (80 bp up to 30 kb), DNA isolated using classical procedures and DNA from reaction mixtures
like the bisulfite method used in methylation analysis.
Advantages:
fast and easy handling
suitable for fragment sizes from 80 bp – 30 kb
complete removal of salts, detergents, nucleotides, mineral oil, primers, enzymes
Product Characteristics:
The optimized Binding Buffer adjust the condition and DNA or DNA fragments will be bound directly onto the surface of a spin filter column during contaminants will be passed through during washing step(s). The buffer volumes are balanced to minimize
pipetting steps. The Buffer P is added directly to the sample, adjusting the binding condition for DNA or DNA fragmentsand
the mixture is applied to the spin filter column. The DNA fragments are bound directly onto the membrane of a spin filter
column. After washing to remove contaminants the DNA or DNA fragments are eluted in a low-salt buffer or ddH2O.
The isolation protocol and all buffers are optimized to provide high yield and purity of the recovered DNA fragments. The
hands-on-time necessary for the whole procedure is reduced to a minimum. The purification process takes less than 10
minutes.
The purified DNA fragments are ready to use in various downstream application such as:
